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phospho erk1  (R&D Systems)


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    R&D Systems phospho erk1
    Phospho Erk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho erk1 - by Bioz Stars, 2026-09
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    Phospho Erk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) <t>ERK2</t> ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    phospho erk1 t202 y204  (R&D Systems)
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) <t>ERK2</t> ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    erk2 t185 y187 detection  (R&D Systems)
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) <t>ERK2</t> ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) <t>ERK2</t> ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) <t>ERK2</t> ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    p erk1  (R&D Systems)
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) <t>ERK2</t> ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    t185 y187  (R&D Systems)
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) <t>ERK2</t> ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
    T185 Y187, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) ERK2 ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Stem Cell Research & Therapy

    Article Title: Investigations into the basal neural-like properties of dental pulp stem cells reveal they possess a functional type 2 muscarinic receptor which regulates quiescence

    doi: 10.1186/s13287-025-04730-7

    Figure Lengend Snippet: m2AChR signalling in dental pulp stem cells is mediated by the MAPK/ERK signalling pathway. A Analysis of expression of genes involved in the MAPK/ERK signalling pathway following stimulation of DPSCs with the M2 agonist (100 µM APE) for 72 h. (i) ERK1 ( MAPK3 ), (ii) ERK2 ( MAPK1 ) and (iii) Proliferating cell nuclear antigen ( PCNA ), Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using unpaired two-tailed t-tests. B Time course of phosphorylation of ERK 1/2 after activation of the dental pulp stem cell m2AChR determined using an in-cell ELISA. Cells cultured in media alone acted as a control (ctrl). M2 agonist = cells cultured in media containing 100 µM APE. Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± SEM from duplicate wells across three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: To evaluate the activation of the extracellular signal-regulated kinases (ERK) 1 & 2, a Phospho-ERK1 (T202/Y204) / ERK2 (T185/Y187) incell ELISA (R&D Systems, UK) was performed.

    Techniques: Expressing, Cell Culture, Control, Two Tailed Test, Phospho-proteomics, Activation Assay, In-Cell ELISA